Journal: The Journal of Neuroscience

Article Title: GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model

doi: 10.1523/JNEUROSCI.4376-14.2015

Figure Lengend Snippet: Increase in p-GluA2 protein levels in COH rat retinae. A, Representative immunoblots showing the changes in p-GluA2 and GluA2 protein expression in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B, Bar chart shows that the average p-GluA2/GluA2 ratio steadily increased with postoperational time. All of the data are normalized to Ctr. n = 6 for all groups. *p < 0.05, **p < 0.01, and ***p < 0.001 vs Ctr. C, Co-IP experiments showing the interactions between GluA2 and p-src, GluA2 and ephrinB2 in normal retinae and retinae with COH. Bands of p-src at the location corresponding to 65 kDa (top) and bands of ephrinB2 at 37 kDa (bottom) were detected in the immunoprecipitates derived using the antibody against GluA2 both in normal and COH retinal extracts (G2w). D, Co-IP experiments showing the interactions of phosphorylated tyrosine with GluA2, and with ephrinB2 in normal retinae and retinae with COH. A band of GluA2 at 110 kDa (top) and of ephrinB2 at 37 kDa (bottom) were detected in the immunoprecipitates derived using a phosphorylated antibody directly against tyrosine in both normal and COH retinal extracts.

Article Snippet: The sections were blocked for 2 h in 6% normal donkey serum, 1% bovine serum (for EphB1 immunostaining, adding 0.2% Triton X-100), dissolved in PBS at room temperature, and then incubated with the following primary antibodies at 4°C for 48 h: monoclonal mouse anti-EphB1 (1:50 dilution; R&D Systems), polyclonal goat anti-EphB2 (1:50 dilution; R&D Systems) or polyclonal rabbit anti-EphB2 (1:300 dilution; Santa Cruz Biotechnology), polyclonal goat anti-ephrinB1 (1:50 dilution; R&D Systems), polyclonal goat anti-ephrinB2 (1:50 dilution; R&D Systems), polyclonal rabbit anti-glutamine synthetase (GS; 1:2000 dilution; Abcam), polyclonal goat anti-CTB (1:2000 dilution; List Biological Laboratories), monoclonal mouse anti-GFAP (1:400 dilution; Sigma-Aldrich), monoclonal mouse anti-Brn-3a (1:100 dilution; Santa Cruz Biotechnology), and polyclonal rabbit anti-GluA2 (1:200 dilution; Alomone Labs).

Techniques: Western Blot, Expressing, Co-Immunoprecipitation Assay, Derivative Assay

Journal: The Journal of Neuroscience

Article Title: GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model

doi: 10.1523/JNEUROSCI.4376-14.2015

Figure Lengend Snippet: Intravitreal injection of PP2 reduces p-src and p-GluA2 levels in EphB2-Fc-injected retinae and retinae with COH. A, Representative immunoblots showing the changes in protein levels of p-src, src, and p-GluA2; and in levels of GluA2 in Ctr-Fc-injected retinae, EphB2-Fc-injected retinae, and retinae with COH (G2w), with or without the addition of PP2. B, Bar chart summarizing the average ratios of p-src/src and p-GluA2/GluA2 under different conditions. All of the data are normalized to control (Ctr-Fc). n = 6 for all the groups.

Article Snippet: The sections were blocked for 2 h in 6% normal donkey serum, 1% bovine serum (for EphB1 immunostaining, adding 0.2% Triton X-100), dissolved in PBS at room temperature, and then incubated with the following primary antibodies at 4°C for 48 h: monoclonal mouse anti-EphB1 (1:50 dilution; R&D Systems), polyclonal goat anti-EphB2 (1:50 dilution; R&D Systems) or polyclonal rabbit anti-EphB2 (1:300 dilution; Santa Cruz Biotechnology), polyclonal goat anti-ephrinB1 (1:50 dilution; R&D Systems), polyclonal goat anti-ephrinB2 (1:50 dilution; R&D Systems), polyclonal rabbit anti-glutamine synthetase (GS; 1:2000 dilution; Abcam), polyclonal goat anti-CTB (1:2000 dilution; List Biological Laboratories), monoclonal mouse anti-GFAP (1:400 dilution; Sigma-Aldrich), monoclonal mouse anti-Brn-3a (1:100 dilution; Santa Cruz Biotechnology), and polyclonal rabbit anti-GluA2 (1:200 dilution; Alomone Labs).

Techniques: Injection, Western Blot

Journal: The Journal of Neuroscience

Article Title: GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model

doi: 10.1523/JNEUROSCI.4376-14.2015

Figure Lengend Snippet: Changes in membrane GluA2 protein expression in retinae with COH. A, Representative immunoblots showing the changes in GluA2 protein levels in the cell membrane component (m-GluA2) in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B, Bar chart summarizing the average densitometric quantification of immunoreactive bands of m-GluA2 at different times. All of the data are normalized to Ctr. n = 6. *p < 0.05 and **p < 0.01 vs Ctr.

Article Snippet: The sections were blocked for 2 h in 6% normal donkey serum, 1% bovine serum (for EphB1 immunostaining, adding 0.2% Triton X-100), dissolved in PBS at room temperature, and then incubated with the following primary antibodies at 4°C for 48 h: monoclonal mouse anti-EphB1 (1:50 dilution; R&D Systems), polyclonal goat anti-EphB2 (1:50 dilution; R&D Systems) or polyclonal rabbit anti-EphB2 (1:300 dilution; Santa Cruz Biotechnology), polyclonal goat anti-ephrinB1 (1:50 dilution; R&D Systems), polyclonal goat anti-ephrinB2 (1:50 dilution; R&D Systems), polyclonal rabbit anti-glutamine synthetase (GS; 1:2000 dilution; Abcam), polyclonal goat anti-CTB (1:2000 dilution; List Biological Laboratories), monoclonal mouse anti-GFAP (1:400 dilution; Sigma-Aldrich), monoclonal mouse anti-Brn-3a (1:100 dilution; Santa Cruz Biotechnology), and polyclonal rabbit anti-GluA2 (1:200 dilution; Alomone Labs).

Techniques: Expressing, Western Blot

Journal: The Journal of Neuroscience

Article Title: GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model

doi: 10.1523/JNEUROSCI.4376-14.2015

Figure Lengend Snippet: Changes in membrane GluA2 protein expression in EphB2-Fc-injected retinae and retinae with COH. A–C, Immunofluorescence labeling showing GluA2 expression profiles (green) in rat retinal vertical slices taken from Ctr-Fc-injected (a1) and EphB2-Fc-injected (b1) retinae 2 weeks after the injection, and from retinae with COH in G2w (c1). a2, b2, and c2, Brn-3a-labeled images (red). a3, b3, and c3, Merged images. D, E, GluA2 expression profiles (green) in rat retinal slices taken from EphB2-Fc-injected retinae, obtained 2 weeks after the injection (Dd1), and from retinae with COH, obtained in G2w (Ee1), when these preparations were intravitreally preinjected with PP2 (100 μm, 2 μl) 3 d before the operation or EphB2-Fc injection. d2, e2, Brn-3a-labeled images (red). d3, e3, Corresponding merged images. Scale bar, 20 μm. F, Representative immunoblots showing the changes in protein levels of m-GluA2 in retinae obtained under different conditions. G, Bar chart showing the average densitometric quantification of immunoreactive bands of m-GluA2 under different conditions. Note that EphB2-Fc injection/IOP elevation induced a significant decrease in m-GluA2 protein levels; preinjection of PP2 reversed the changes. All of the data are normalized to Ctr-Fc. n = 6 for all groups. *p < 0.05 and **p < 0.01 vs Ctr-Fc.

Article Snippet: The sections were blocked for 2 h in 6% normal donkey serum, 1% bovine serum (for EphB1 immunostaining, adding 0.2% Triton X-100), dissolved in PBS at room temperature, and then incubated with the following primary antibodies at 4°C for 48 h: monoclonal mouse anti-EphB1 (1:50 dilution; R&D Systems), polyclonal goat anti-EphB2 (1:50 dilution; R&D Systems) or polyclonal rabbit anti-EphB2 (1:300 dilution; Santa Cruz Biotechnology), polyclonal goat anti-ephrinB1 (1:50 dilution; R&D Systems), polyclonal goat anti-ephrinB2 (1:50 dilution; R&D Systems), polyclonal rabbit anti-glutamine synthetase (GS; 1:2000 dilution; Abcam), polyclonal goat anti-CTB (1:2000 dilution; List Biological Laboratories), monoclonal mouse anti-GFAP (1:400 dilution; Sigma-Aldrich), monoclonal mouse anti-Brn-3a (1:100 dilution; Santa Cruz Biotechnology), and polyclonal rabbit anti-GluA2 (1:200 dilution; Alomone Labs).

Techniques: Expressing, Injection, Immunofluorescence, Labeling, Western Blot

Journal: The Journal of Neuroscience

Article Title: GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model

doi: 10.1523/JNEUROSCI.4376-14.2015

Figure Lengend Snippet: Activation of EphB2/ephrinB2 reverse signaling induces GluA2-containing AMPA receptor trafficking. A, B, Representative traces showing the changes in amplitude of evoked EPSCs, mediated by AMPA receptors, recorded from four different RGCs in retinal slices at holding potentials of +40 and −60 mV with (A) or without (B) spermine in the pipette solution. The eyes were intravitreally injected respectively with control-Fc (Ctr-Fc) and EphB2-Fc (2 μl, 1 μg/μl), and the recordings were made 5–7 d after the injections. The current amplitudes were all normalized to that of Ctr-Fc at −60 mV. C, Bar chart showing the average ratios of current amplitudes at +40/−60 mV in RGCs obtained from Ctr-Fc- and EphB2-Fc-injected retinae with or without spermine in the pipette solution. *p < 0.05 vs Ctr-Fc.

Article Snippet: The sections were blocked for 2 h in 6% normal donkey serum, 1% bovine serum (for EphB1 immunostaining, adding 0.2% Triton X-100), dissolved in PBS at room temperature, and then incubated with the following primary antibodies at 4°C for 48 h: monoclonal mouse anti-EphB1 (1:50 dilution; R&D Systems), polyclonal goat anti-EphB2 (1:50 dilution; R&D Systems) or polyclonal rabbit anti-EphB2 (1:300 dilution; Santa Cruz Biotechnology), polyclonal goat anti-ephrinB1 (1:50 dilution; R&D Systems), polyclonal goat anti-ephrinB2 (1:50 dilution; R&D Systems), polyclonal rabbit anti-glutamine synthetase (GS; 1:2000 dilution; Abcam), polyclonal goat anti-CTB (1:2000 dilution; List Biological Laboratories), monoclonal mouse anti-GFAP (1:400 dilution; Sigma-Aldrich), monoclonal mouse anti-Brn-3a (1:100 dilution; Santa Cruz Biotechnology), and polyclonal rabbit anti-GluA2 (1:200 dilution; Alomone Labs).

Techniques: Activation Assay, Transferring, Injection

Journal: Addiction biology

Article Title: Optogenetic brain-stimulation reward: A new procedure to re-evaluate the rewarding versus aversive effects of cannabinoids in dopamine transporter-Cre mice

doi: 10.1111/adb.13005

Figure Lengend Snippet: Cannabinoid receptor binding affinities of cannabinoids tested in this study

Article Snippet: Dual-labeling IHC was performed using a CB 2 R antibody (Alomone, #ACR-002, 1:250) and an anti-tyrosine hydroxylase (anti-TH) monoclonal antibody (1:500; Millipore, Billerica, MA, USA).

Techniques: Binding Assay

Journal: International Journal of Molecular Sciences

Article Title: Transplantation of Neural Precursors Derived from Induced Pluripotent Cells Preserve Perineuronal Nets and Stimulate Neural Plasticity in ALS Rats

doi: 10.3390/ijms21249593

Figure Lengend Snippet: List of primary antibodies.

Article Snippet: Anti-Ryanodine Receptor 3 , Ryanodine Receptor 3/ICC , Rabbit polyclonal , 1:100 , Alomone Labs.

Techniques: Marker

Journal: Nature Communications

Article Title: Plakophilin-2 is required for transcription of genes that control calcium cycling and cardiac rhythm

doi: 10.1038/s41467-017-00127-0

Figure Lengend Snippet: Structural changes consequent to PKP2 deletion. a STORM-acquired images of Cav1.2 ( green ) and RyR2 ( purple ) in a single myocyte. b Analysis of RyR2/Cav1.2 overlapping area in transverse ( left ) and longitudinal ( right ) clusters. n = 1335 and 1285 clusters from 30 control and 25 PKP2-cKO cardiomyocytes at 21 dpi, respectively. t -test, * p < 0.05 and ** p < 0.01 vs. control. c 2D-EM image of PKP2-cKO ventricular tissue at 21 dpi showing a dyadic structure. Scale bar = 200 nm. d Boundaries of the jSR ( red ) and T-tubule ( blue ) membranes, detected from the dyad in the dotted square in c . e Acquisition of distances from each point in the T-tuble membrane to its closest neighbor in the jSR ( green lines ). f Close-up of the region inside the dashed square in e . All distances measured within a dyad were averaged to obtain the “average distance” for that dyad (expressed in nm). g Comparison of average data collected from one control ( n = 30 dyads) and 2 PKP2-cKO 21 dpi samples ( n = 41 dyads for both). Student’s t -test * p < 0.001 vs. control. h Spatial orientation of the T-tubular network, obtained from segmentation and analysis of a volume of 15 × 12 × 0.8 µm 3 dimensions obtained by Serial Block-Face Scanning Electron Microscopy analysis. See also Supplementary Video and “Methods”. The angle of the T-tubular skeleton is color-coded ( bottom left ) from −90° in magenta to +90° in red ; relative to the longitudinal axis of the cell ( light blue ; 0°). i Histogram of orientations (from h ), showing a strong preference for zero-degree orientation, as expected from a non-failing heart (see ref. )

Article Snippet: The following primary antibodies were used: monoclonal mouse anti-PKP2 (K44262M; Biodesign International, Meridian Life Sciences), monoclonal mouse anti-AnkyrinB (N105-17; BioLegend), polyclonal rabbit anti-Cav1.2 (ACC-003; Alomone), polyclonal rabbit anti-CASQ2 (PA1-913; ThermoFisher), monoclonal mouse anti-RyR2 (MA3-916; ThermoFisher), polyclonal rabbit anti-RyR2 phospho serine-2808 and anti-RyR2 phospho serine-2814 (A010-30 and A010-31 respectively; Badrilla), monoclonal mouse anti-TriadinT32 (MA3-927; ThermoFisher), polyclonal rabbit anti-NCX (sc-32881, SantaCruz), polyclonal rabbit anti-SERCA2 (PA5-29380; ThermoFisher), monoclonal mouse anti-phospholamban (sc-393990; SantaCruz), polyclonal rabbit anti-phospholamban phospho serine-16 (sc-12963; SantaCruz) and phospho threonine-17 (sc-17024; SantaCruz), GAPDH (G109A; Fitzerald) monoclonal mouse anti-Cx43 (Clone 4E6.2, Millipore), polyclonal rabbit anti-β-catenin (C2206; Sigma), monoclonal mouse anti-JPH2 (sc-37086, SantaCruz), monoclonal mouse anti-Bin1 (Clone 99D; Sigma), polyclonal rabbit anti-PKC and anti-phospho PKC (#2056 and #9375; Cell Signaling), polyclonal rabbit anti-CaMKII (PA5-22168; ThermoFisher), monoclonal mouse anti-CaMKII phospho threonine-286 (MA1-047; ThermoFisher), polyclonal rabbit Desmocollin-2 (ab72792; Abcam), polyclonal rabbit anti-Nav1.5 (S0819; Sigma).

Techniques: Control, Membrane, Comparison, Blocking Assay, Electron Microscopy

Journal: The Journal of comparative neurology

Article Title: Neurons with diverse phenotypes project from the caudal to the rostral nucleus of the solitary tract

doi: 10.1002/cne.24501

Figure Lengend Snippet: ANTEROGRADE VIRUS INJECTIONS † , ‡ , § , ¶

Article Snippet: Rabbit Anti-P2×2 polyclonal , Peptide (C)SQQDSTSTDPKGLAQL, corresponding to amino acid residues 457–472 of rat P2×2 Receptor (Accession {"type":"entrez-protein","attrs":{"text":"P49653","term_id":"1352688","term_text":"P49653"}} P49653 ). Intracellular, C-terminus. , Alomone APR-003 , RRID: AB_2040054 , 1:10000.

Techniques: Plasmid Preparation, Injection, Immunohistochemistry

Journal: The Journal of comparative neurology

Article Title: Neurons with diverse phenotypes project from the caudal to the rostral nucleus of the solitary tract

doi: 10.1002/cne.24501

Figure Lengend Snippet: PRIMARY ANTIBODIES

Article Snippet: Rabbit Anti-P2×2 polyclonal , Peptide (C)SQQDSTSTDPKGLAQL, corresponding to amino acid residues 457–472 of rat P2×2 Receptor (Accession {"type":"entrez-protein","attrs":{"text":"P49653","term_id":"1352688","term_text":"P49653"}} P49653 ). Intracellular, C-terminus. , Alomone APR-003 , RRID: AB_2040054 , 1:10000.

Techniques: Isolation, Fluorescence, Variant Assay

Journal: The Journal of comparative neurology

Article Title: Neurons with diverse phenotypes project from the caudal to the rostral nucleus of the solitary tract

doi: 10.1002/cne.24501

Figure Lengend Snippet: Injection site for case ISM26 with an injection of an AAV1.hSynap.eGFP virus in cNST centered near the level of obex medial to the solitary tract. a. Confocal images near the injection site center. The upper panel shows the virus alone and the lower panel shows immunostaining for P2×2 which marks the axons and terminals of a subset of primary afferent vagal neurons, and choline acetyltransferase (CHAT), which stains preganglionic parasympathetic neurons in the dorsal motor nucleus of the vagus and motor neurons in the hypoglossal nucleus. Dotted lines denote the borders of the area postrema and central canal in both panels. b. Series of low-power photomicrographs of injection site viral labeling alone (left) and viral labeling superimposed on darkfield images (right) arranged from caudal (top) to rostral (bottom). The second section from the top corresponds to the confocal image in (a). Sections are separated by ~250µm, except for the two most rostral, which are adjacent 40µm sections. Although some labeled neurons are apparent outside NST, virtually all of these are confined to the dorsal motor nucleus of the vagus and hypoglossal nucleus, which only send axons to peripheral targets. The arrowhead in the rostral-most section points to the (caudal/intermediate) central subnucleus, which is virtually devoid of afferent labeling. The boxed region containing the central subnucleus is shown with the brightness and contrast enhanced in the inset (gray arrow). Abbreviations- AP: area postrema, C: central canal, ce: central subdivision, CHAT: choline acetyltransferase, m: medial subdivision, st- solitary tract, X: dorsal motor nucleus of the vagus, XII: hypoglossal nucleus.

Article Snippet: Rabbit Anti-P2×2 polyclonal , Peptide (C)SQQDSTSTDPKGLAQL, corresponding to amino acid residues 457–472 of rat P2×2 Receptor (Accession {"type":"entrez-protein","attrs":{"text":"P49653","term_id":"1352688","term_text":"P49653"}} P49653 ). Intracellular, C-terminus. , Alomone APR-003 , RRID: AB_2040054 , 1:10000.

Techniques: Injection, Immunostaining, Labeling

Journal: The Journal of comparative neurology

Article Title: Neurons with diverse phenotypes project from the caudal to the rostral nucleus of the solitary tract

doi: 10.1002/cne.24501

Figure Lengend Snippet: Confocal photomicrographs (maximum intensity projections, z=2µm intervals) of rNST anterograde labeling arising from the cNST injection shown in Figure 4. Panels a1–a3 depict viral label in three sections arranged from caudal (a1) to rostral (a3). Panel a2 is from a mid-level of rNST and denotes the approximate locations of rNST subdivisions. Panels b1–b3 show the same three sections with immunostaining for P2×2 and CHAT. Anterograde labeling is most prominent in the ventral and medial subdivisions, i.e., ventral and medial to the P2×2 field, which largely corresponds to the (rostral) central subdivision. Moreover, the medial projections overlap extensively with CHAT-stained preganglionic parasympathetic neurons. Panels c1–c3 show high-magnification photomicrographs (maximum intensity projection, z=1um intervals) from a nearby section at the mid-NST level. C1: a field overlapping the P2×2 staining and ventrally adjacent region. Some varicosities are present in the P2×2 field but they are much denser ventrally. C2: medial rNST. Numerous varicosities intermingle with the CHAT neurons. C3: Magnified image of the area denoted by the white square; this flattened image sed just 9/41 z levels in C2. A few varicosities appear to appose dendrites or somata (arrowheads). Abbreviations- MV: medial vestibular nucleus, SpV: spinal vestibular nucleus, st: solitary tract, RF: reticular formation. rNST subdivisions- m: medial, c: central, v: ventral, l: lateral.

Article Snippet: Rabbit Anti-P2×2 polyclonal , Peptide (C)SQQDSTSTDPKGLAQL, corresponding to amino acid residues 457–472 of rat P2×2 Receptor (Accession {"type":"entrez-protein","attrs":{"text":"P49653","term_id":"1352688","term_text":"P49653"}} P49653 ). Intracellular, C-terminus. , Alomone APR-003 , RRID: AB_2040054 , 1:10000.

Techniques: Labeling, Injection, Immunostaining, Staining

Journal: bioRxiv

Article Title: Paladin is a PI(4,5)P 2 phosphoinositide phosphatase that regulates endosomal signaling and angiogenesis

doi: 10.1101/2020.02.11.943183

Figure Lengend Snippet: Paladin regulates VEGFR2 signaling. (a–d) Signaling downstream of VEGF-A/VEGFR2 assessed in HDMEC, untreated, transfected with non-targeting siRNA (NT/cntrl), or with two different PALD1 -targeting siRNAs (KD#1 and KD#2), and treated with VEGF-A for 0-20 min. Immunoblotting of cell lysates for phosphorylated (p) VEGFR2 (pY1175), total VEGFR2, phosphorylated Erk1/2 (pT202 and pY204), and total Erk. Actin served as loading control. PALD1 knockdown was verified by blotting for Paladin. Quantification of pVEGFR2 (normalized to total VEGFR2) (b) and pErk1/2 (normalized to total Erk1/2) (d). Mean±SEM, two-way ANOVA. n=3. (e–j) Immunoblotting on total heart lysates from Pald1 +/+ and Pald1 -/- mice, tail-vein injected with VEGF-A or PBS for the indicated time points, for Paladin, phosphorylated and total levels of VEGFR2, phospholipase Cγ (PLCγ), Erk1/2, and Akt, and β 2-microglobulin ( β 2-MG, loading control) (e). Quantification of total VEGFR2 levels normalized to total loading control (f), pT202/pY204 Erk1/2 normalized to Erk1/2 (g) pY783 PLCγ normalized to PLCγ (h), pS473 Akt normalized to Akt (i), pY416 Src family kinase (SFK) normalized to pY527 SFK (j). Blot for total c-Src is shown in Suppl. Figure 3c. Mean±SEM, multiple t -test (Holm-Sidak for panel e), two-way ANOVA. n=4-5.

Article Snippet: Rabbit anti-paladin (1:1000; Atlas Antibodies, HPA017343), rabbit anti-phospho-VEGFR2 pY1175 (1:1000, Cell Signaling, 2478), rabbit anti-VEGFR2 (1:1000, Cell Signaling, 2479), rabbit anti-phospho-PLCγ pY783 (1:1000, Invitrogen, 44-696G), rabbit anti-PLCγ (1:1000, Cell Signaling, 2822), rabbit anti-phospho-Erk1/2 pThr202/pTyr204 (1:1000, Cell Signaling, 4377), rabbit anti-Erk1/2 (1:1000, Cell Signaling, 9102), rabbit anti-phospho-Akt pSer473 (1:1000, Cell Signaling, 4060), rabbit anti-Akt (1:1000, Cell Signaling, 9272), rabbit anti-phospho-Src pTyr416 (1:1000, Cell Signaling, 6349), rabbit anti-phospho-Src pTyr527 (1:1000, Cell Signaling, 2105), rabbit anti-Src (1:1000 Cell Signaling, 2123) and or goat anti-actin (1:1000, Santa Cruz, sc1615).

Techniques: Transfection, Western Blot, Control, Knockdown, Injection